Thus, the aim of this study was to

Thus, the aim of this study was to investigate chromatographic behavior of geometric isomers of olopatadine and benzalkonium chloride in HILIC mode with the aim to define optimal chromatographic
conditions suitable for their determination in eye drops. In order to get the most suitable chromatographic conditions,design of experiments (DoE) methodology was employed.
On the basis of the obtained mathematical models, an appropriate interpretation of chromatographic behavior of investigated analytes in the mixture was carried out. The multi-objective robust
optimization is performed aiming to achieve maximal retention of the first eluting peak, satisfactory separation of the critical peak pair and satisfactory robustness of the obtained separation
criterion in terms of all investigated factors. The measurement of the robustness is performed calculating the partial derivatives of the response function with respect to the investigated factors.
Grid point search methodology was applied for identification of the optimal chromatographic conditions

結果 (中文) 1:

因此，本研究的目的是探讨奥洛他定和苯扎氯铵 HILIC 模式，目的是确定最优几何异构体的色谱行为色谱条件适合他们滴眼液中的决心。 为了得到最合适的色谱条件，采用设计实验 (DoE) 方法。根据得到的数学模型，进行了混合物中调查分析物的色谱行为适当解释。 多目标鲁棒旨在达到最大保留的第一次的洗脱峰、 令人满意的分离临界峰值对和令人满意的鲁棒性得到分离进行优化在所有调查的因素方面的标准。 鲁棒性被测量计算与调查因素的响应函数的偏导数。网格点搜索方法被申请鉴定的最佳色谱条件

結果 (中文) 3:

Thus，the aim并明确study was to investigate chromatographic behavior明确geometric isomers明确olopatadine and benzalkonium chloride in HILIC mode with the aim to define optimal chromatographicconditions suitable在their determination in eye drops . In order to the most搞定suitable chromatographic conditions，design明确experiments（DoE）methodology was employed .论the basis明确the obtained mathematical models，an appropriate interpretation明确chromatographic behavior明确investigated analytes in the mixture was carried out . The multi - objective robustoptimization is performed aiming to achieve maximal retention明确the first eluting peak，satisfactory separation明确the critical peak pair and satisfactory robustness明确the obtained separationcriterion in terms明确all investigated factors . The measurement明确the robustness is performed calculating the partial derivatives明确the response function with respect to the investigated factors .Grid point search methodology was applied在identification明确the optimal chromatographic conditions