Poor retention of polar and/or ioni

Poor retention of polar and/or ionic compounds or unsuitable peak shape of polar substances in reversed-phase liquid chromatography (RP-LC) in many cases was solved by application of hydrophilic
interaction liquid chromatography (HILIC). Generally, HILIC separation is carried out on polar columns (such as bare silica) with mobile phases consisting of an aqueous–organic
mixture (.50% of organic solvent, usually acetonitrile). Aqueous phase usually contains buffers such as ammonium acetate or ammonium formate. Such a mobile phase composition is compatible with mass detection, which could be considered an additional advantage of the HILIC method. Significance of the
HILIC method could be confirmed through the increasing number of papers dealing with its application in drug analysis. Some of the most recently published are references (1–6). Taking into account analytes’ characteristics, it was challenging to investigate behavior of geometric isomers Z and E of olopatadine in HILIC mode. Significance of this investigation is especially important
due to the fact that one isomer is active substance and another is impurity. Because benzalkonium chloride is a typical additive in pharmaceutical formulations of this drug, it was included in
the investigation as well. The chemical structures of the analyzed substances are presented in Figure

結果 (中文) 1:

较差的极性和 （或） 离子化合物的保留或不合适的峰形的极性物质在反相液相色谱法 (RP-LC) 在许多情况下，解决了应用程序的亲水性互动高效液相色谱法 (HILIC)。 一般来说，HILIC 分离是对进行极地列 （如裸硅） 与流动相组成的水性有机混合 (.50%的有机溶剂，通常乙腈)。 水相通常包含缓冲区，醋酸铵或甲酸铵等。 这种流动相的组成是符合质量检测，可以考虑 HILIC 方法一个额外的优势。 意义HILIC 方法能证实通过越来越多的文件处理及其在药物分析中的应用。 最最近发表一些参考 (1-6)。 考虑到帐户分析物的特性，它在挑战调查的几何异构体 Z 和 E olopataine 在 HILIC 模式下的行为。 这次调查的意义是特别重要一个异构体是活性物质和另一种是杂质。 因为苯扎氯铵是一种典型的添加剂在医药制剂的这种药物，它被列入以及进行调查。 分析物质的化学结构介绍了图

結果 (中文) 3:

Poor retention明确polar and / or ionic compounds or unsuitable peak shape明确polar substances in reversed - phase liquid chromatography（反相- LC）in many cases was solved by application明确hydrophilicinteraction liquid chromatography（HILIC ). Generally，HILIC separation is carried out on polar columns（such as bare silica）with mobile phases consisting明确an aqueous–organicmixture (. 50% organic solvent明确，usually acetonitrile ). Aqueous phase usually contains buffers such as ammonium acetate or ammonium formate . Such a mobile phase composition is compatible with mass detection，which could执意considered an additional advantage明确the HILIC method . Significance明确theHILIC method could执意confirmed through the increasing number明确papers dealing with其application in drug analysis . Some明确the most recently published are references（1–6 ). Taking into account analytes’，characteristics it was challenging to investigate behavior明确geometric isomers Z and E明确olopatadine in HILIC mode . Significance并明确investigation is especially importantdue to the fact即在isomer is active substance and another is impurity . Because benzalkonium chloride is a typical additive in pharmaceutical formulations明确，并drug it was included inthe investigation as well . The chemical structures明确the analyzed substances are presented in Figure